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當(dāng)前位置:主頁(yè) > 產(chǎn)品中心 > 相關(guān)檢測(cè)試劑 > ELISA檢測(cè)試劑盒 >Alpco 80-INSPO-E01 ALPCO C-peptide ELISA檢測(cè)試劑盒,25µl,96孔

Alpco 80-INSPO-E01 ALPCO C-peptide ELISA檢測(cè)試劑盒,25µl,96孔

簡(jiǎn)要描述:產(chǎn)品英文名稱:C-peptide ELISA Kit
產(chǎn)品中文名稱:C-肽ELISA檢測(cè)試劑盒
貨號(hào):80-CPTHU-E01.1
廠家:美國(guó)Alpco公司
品牌:Alpco
規(guī)格:96T/盒
產(chǎn)品應(yīng)用:C-肽ELISA檢測(cè)
保存溫度: 2-8℃
貨期:2~5天
詳情請(qǐng)。

  • 產(chǎn)品型號(hào):80-CPTHU-E01.1
  • 廠商性質(zhì):代理商
  • 更新時(shí)間:2016-12-27
  • 訪  問(wèn)  量:1713
詳細(xì)介紹
品牌ALPCO

產(chǎn)品英文名稱:C-peptide ELISA Kit
產(chǎn)品中文名稱:C-肽ELISA檢測(cè)試劑盒

§ 檢測(cè)原理:ALPCO C-肽ELISA是一個(gè)夾層式免疫檢測(cè)試劑盒。96孔微孔板涂有一層C-肽特異性單克隆抗體。將標(biāo)準(zhǔn)物,對(duì)照品和樣品一并添加到微型板小孔中與檢測(cè)緩沖液混合。然后將微孔板放在搖床上室溫溫育1小時(shí),700-900轉(zhuǎn)/分鐘。*次溫育結(jié)束,用清洗緩沖液清洗小孔并烘干。添加結(jié)合物(配制方法參照說(shuō)明書),微孔板放在搖床上第二次室溫溫育1小時(shí),700-900轉(zhuǎn)/分鐘。添加TMB作用底物,放在微孔板搖床上室溫溫育15分鐘,700-900轉(zhuǎn)/分鐘。第三次溫育結(jié)束,添加反應(yīng)終止液,調(diào)節(jié)分光光度計(jì)到450nm測(cè)定OD值。由于發(fā)光強(qiáng)度與樣品中C-肽含量成比例,因此可以根據(jù)OD值確定C-肽含量。

§ 檢測(cè)過(guò)程:各添加25μl標(biāo)準(zhǔn)物,對(duì)照物和樣品→加50µl檢測(cè)緩沖液→微孔板搖床室溫溫育1小時(shí),700-900rpm→清洗緩沖液清洗6次→加100µl結(jié)合物(配制方法參照說(shuō)明書)→微孔板搖床室溫溫育1小時(shí),700-900rpm→清洗緩沖液清洗6次→添加100µl TMB作用底物→微孔板搖床室溫溫育15分鐘,700-900rpm→添加100µl反應(yīng)終止液→分光光度計(jì)450nm讀數(shù)

ALPCO C-peptide ELISA kit英文簡(jiǎn)述:
The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time. 

INTENDED USE:The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.

PRINCIPLE OF THE ASSAY:The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.

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